A multiple-sequence alignment of the kit's HIV-1 subtype reference sequences is presented below in Clustal format:

Note that half the HMA primers were reversed and complemented to match the strand orientation of the alignment.  These primers are indicated within their sequence names by "REVCOMP."  Subtypes are indicated after the hyphens.

CLUSTAL W (1.74) multiple sequence alignment


sf170               ATGGGACCAGAGTCTAAAGCCATGTGTAAAGTTAACCCCTCTCTGCGTTA
br20                ATGGGATCAAAGCCTAAAGCCTTGTGTAAAGTTAACCCCACTCTGTGTTA
th14                ATGGGATCAAAGCCTAAAGCCATGTGTAAAATTAACCCCACTCTGTGTTA
sf162               ATGGGATCAAAGTCTAAAGCCATGTGTAAAGTTAACCCCACTCTGTGTTA
ma959               ATGGGATCAAAGCCTAAAGCCATGTGTAAAGTTGACCCCACTCTGTGTCA
zm18                ATGGGATCAAAGCTTAAAGCCATGTGTAAAGTTGACCCCACTCTGTGTCA
in868               ATGGGATCCAAGCCTGAAGCCATGTGTAAAGTTGACCCCACTCTGTGTCA
ug21                ATGGGATCAAAGCCTAAAACCATGTGTAAAATTACCCCCACTCTGTGTCA
ug46                --------------------------------------------------
th22                ATGGGATCAAAGTCTAAAGCCATGTGTAAAGTTAACTCCTCTCTGTGTTA
th06                ATGGGATCAAAGTCTAAAGCCATGTGTAAAGTTAACTCCTCTCTGCGTTA
bz162               ATGGGATCAAAGCCTAAAGCCATGTGTGAAGTTAACCCCACTCTGTGTTA
bz163               ATGGGATCAAAGCCTAAAGCCATGTGTGAAGTTAACCCCACTCTGTGTTA
ru131               ATGGGATGAAAGCCTAAAGCCATGTGTAAAGTTAACCCCTCTCTGTGTTA
lbv21               ATGGGATGAAAGCCTAAAGCCATGTGTAAAGTTAACCCCTCTCTGTGTTA
vi525               ATGGGATGAAAGCTTAAAGCCATGTGTAGAGCTAACCCCTCTCTGTGTTA
vi557               --------------------------------------------------
se9173              ATGGGATGAAAGCTTAAAGCCATGTGTAAAGATAACCCCTCTCTGTGTCA
se9280              ATGGGATGAAAGCTTAAAGCCATGTGTAAAGATAACCCCTCTATGTGTCA
primer_ed5          ATGGGATCAAAGCCTAAAGCCATGTG------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               CTTTAAATTGTAGCCAT---AACATCACCACC------------------
br20                CTTTAAATTGCATTGATTTGAAT---------------------------
th14                CTTTAAACTGCACTGATTTGAGG---------------------------
sf162               CTCTACATTGCACTAATTTGAAG---------------------------
ma959               CTTTAAATTGTAGCAATGCTAAC---------------------------
zm18                CTTTAGAATGTGGAAATGTTAAT---------------------------
in868               CTTTAGAATGTAGAGAGGTTAAT---------------------------
ug21                CTTTAAACTGCACTGAATGGAAG---------------------------
ug46                --------------------------------------------------
th22                CTTTAAATTGTACCAATGCTAATTTGATCAAT------------------
th06                CTTTAAATTGTACCAATGCTAAAGTGACCAAT------------------
bz162               CTTTAAATTGTACTGATGCCATC---------------------------
bz163               CTTTAAATTGTACTAATGCCATT---------------------------
ru131               CTTTAAACTGTAGAAATGTAAGCTGTACTAATTGTACTAAGGGAAAC---
lbv21               CCTTAAACTGTACTAAT------------------GCAATCGCTAATGTA
vi525               CTTTAAACTGTACTAAGGTAACCTACAATAGCACTGCAGCCAACAATAGA
vi557               --------------------------------------------------
se9173              CTTTAAATTGTAGCGAC------------------------------GTC
se9280              CTTTAAATTGTAGTAAC------------------------------ATC
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               ---------------CATAACATCACCACCAACAACCCCAATATCACCAA
br20                ---------AATAGTACTAACAACAATAATAGTAGTGGGGTAAAAACAGG
th14                ---------AATGCTACTAATACCAGTAGTACA-----------------
sf162               ---------AATGCTACTAATACCAAGAGTAGTAAT------TGGAAAGA
ma959               ------------------AACACTGCTACCAATAATGTAACTGCTACCAA
zm18                ------------------------------------GTTACCCATGAGAA
in868               ------------------GAT------ACTAGAAATGGGGTTAATGTTAC
ug21                ---------AATGCCACCACTAATGCCACTAATGAGGGCATA--------
ug46                --------------------------------------------------
th22                ---------------GTCAATAACATAACCAATGTCTCTAACATAATAGG
th06                ---------------GTCAGTAATACAATCAATGCCCCTGACATA---GG
bz162               ---------AATAGCACT------AAAAACAGCACC---------CTGAT
bz163               ---------AATGCCACTGCCATTCAAGACGGGGAC---------CTGAA
ru131               ------------------------------------AACAATAGAACTGT
lbv21               ACCAACAGCAATAAGACTAGTAACAACAGCAGCCTTGGGAATAGCACTGT
vi525               TCATCCACTGTCACCACCCCTACTAGTACTGTAACCAACAATAGCACTGT
vi557               --------------------------------------------------
se9173              AATAGTAACAATAGTACAGATAGTAATAGTAGTGCAAGCAACAATAGTCC
se9280              ACTAGTAACAGTAATACAACTAGTAACAGTAGTGTAAGT------AGTCC
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               ATACATGGAAGGAGAAATAAAGAACTGCTCTTACAATATGACCACAGAAC
br20                GATAGACAAGGGAGAGATAAAAAACTGCTCTTTCAATACCACCACAAGTG
th14                -ATGGAGGGAGGAGAAATAAAAAACTGCTCTTTCAATATCACCACGAGCA
sf162               GATGGACAGAGGAGAAATAAAAAATTGCTCTTTCAAGGTCACCACAAGCA
ma959               TAATGTAACCAGTGACATGAAAAATTGTTCTTTCAATGCAACCACAGAAT
zm18                TAGCACGAAGGGGGAAATGAAAAATTGCTCTTTCAATGCAACCACAGAAC
in868               CAATAATGGGGAAGAAATAAAAAATTGCTCTTTCAATGCAACCACAGAAA
ug21                -------------GGAATGAAAAACTGCTCTTTC---------ACAGAAG
ug46                --------------------------------------------------
th22                AAATATAACAGATGAAGTAAGAAACTGTACTTTTAATATGACCACAGAAA
th06                AAATATAACAGATGAAGTAAGAAACTGTTCTTTTAATATGACCACAGAAC
bz162               GGAAGAGTCAGGGGCAATACAAAACTGTTCTTTCAATATGACCACAGAAG
bz163               GGAAGGACCAGGGGCAATACAAAACTGTTCTTTCAATGTGACCATGGAAG
ru131               GGAAGGCAAGGACGAAATGAAAAACTGCTCTTTCAATGTAACTACAGAAA
lbv21               GAGTAGCATAGAAGAAATGAAAAACTGCTCTTTCAATATAACCACAGAAG
vi525               GAGTGGCAAAGAAGAAATGACAAACTGCTCTTTCAATGTAACCACAGAAC
vi557               --------------------------------------------------
se9173              T---------GAAATCATGAAAAACTGCTCTTTCAATGTAACTACAGAAA
se9280              T---------GATATCATGACAAACTGCTCTTTCAATATAACTACAGAAA
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               TAAGGGATAAGAAACAGAAAGTGTATTCACTTTTTTATAAACTTGATGTA
br20                TAAAAGATAAGGAGAAGAAGGAATATGCTCTCTTCTATAATCTTGATGTA
th14                TAAAAACTAAGGTA---AAAGACTATGCACTTTTTTATAAACTTGATGTA
sf162               TAAGAAATAAGATGCAGAAAGAATATGCACTTTTTTATAAACTTGATGTA
ma959               TAAGAGATAAGAGACAGAAAGTGTATGCACTTTTTTATAAACTTGATATA
zm18                TAAAAGATAAAAAACAGAGAGTGTATGCACTTTTTTATAAACTTGATATA
in868               TAAGAGATAGGAAGCAGACAGTGTATGCACTTTTTTATAGACTTGATATA
ug21                TAAGAGATAAGAAGAAGCAAGCATATGCACTTTTTTATAAGCTTGATGTG
ug46                --------------------------------------------------
th22                TAAGAGATAAACAGCAGAAGGTCCATGCACTTTTTTATAGGCTTGATATA
th06                TAAGAGATAAGAAGCAGAAGGTCCATGCACTTTTTTATAAGCTTGATATA
bz162               TAAGAGATAAGCAGAAGAGAGTACATGCACTTTTTTATAAACTTGATATA
bz163               TAAGAGATAAGCAGCAGAAAGTACATGCACTGTTT-ATAGACTTGATATA
ru131               TAAGAGATAAGAAAAAGACAGAATACGCGCTTTTCTATAAACTTGATGTA
lbv21               TAAGAGATAAGAGTAAGAAAGAATACGCTCTTTTCTATAGACTTGATGTG
vi525               TAAGAGATAAGCAGAAGGCAGAATACGCGCTTTTCTATAAACTTGATGTA
vi557               --------------------------------------------------
se9173              TAAGAAATAAAAGGAAGCAAGAATACGCGCTTTTCTATAGACAAGATGTA
se9280              TAAGAAATAAAAGGAAGCAAGAGTACGCGCTTTTCTATAGACAAGATGTA
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               GTACCAATTGATAAAAATAATGGTAATAACAATATAAGGACTCAGTATAG
br20                GTACAAAT---------AGGTAATGATAA------TACTAGC---TATAG
th14                GTACCAAT---------AGATAATGATAA------TACCAGC---TATAG
sf162               GTACCAAT---------AGATAATGATAA------TACAAGC---TATAA
ma959               GTACCACTTAATGAGAAGGATAACAGCAG------CTCTGGAGAATATAG
zm18                GTACCACTTAATGAGAATAACAACTCTAGTGAGGACTCTAGTGAGTATAG
in868               GTACCACTTGATAATAAGAAGAAGAACA---ACTCTAGTGAGTATTATAG
ug21                GTACAAATGAATG---ATGATAATAGTAC------TAATACCAGTTATAG
ug46                --------------------------------------------------
th22                GTACAAAT---------GCATAATGAAAA------TAGTAGTGAGTATAG
th06                GTACAA------------------GTTAA------TAGTAGTGAGTACAG
bz162               GTACCAAT---------CAATGGCAGTAG------AACATCA---TACAG
bz163               GTACCAATTAGCAATGGAAGAAATGGCAG------TGGAGACTACTACAG
ru131               GTACCAATTA------ATGATGAAAGTAG------TAATAGTACTTATAG
lbv21               GTACCAATTAATGGTAATGAAAATAGTAG------TACAACTGATTATAG
vi525               GTACCAAT---------TGATAATAATAG------AAATGATAGTTATAG
vi557               --------------------------------------------------
se9173              GTACCAAT---------TAATAGTGACAA------TAAAAGT---TATAT
se9280              GTACCAAT---------TGATAGTAACAA------TAAAAAT---TATAT
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               ATTAATAAATTGTAATACCTCAGCCATTACACAGGCTTGTCCAAAAGTAT
br20                ATTGACAAGTTGTAATACCTCAGTCATTACCCAGGCCTGTCCAAAGGTAA
th14                GTTGATAAATTGTAACACCTCAGTCATTACACAAGCTTGTCCAAAGGTAT
sf162               ATTGATAAATTGTAACACCTCAGTCATTACACAGGCCTGTCCAAAGGTAT
ma959               ATTAATAAATTGCAGTACCTCAACTGTAACACAAGCCTGTCCAAAGGTCT
zm18                ATTAATAAATTGTAATACCTCAGCCATAACACAAGCCTGTCCAAAGGTCA
in868               ATTAATAAATTGTAATACCTCAGCCATAACACAAGCCTGTCCAAAGGCCA
ug21                ATTAATAAATTGTAATGCCTCAACCATTACACAGGCGTGTCCAAAGATAT
ug46                --------------------------------------------------
th22                GTTAATAAATTGTAATACTTCAGTCATTAAGCAGGCTTGTCCAAAGATAT
th06                GTTAATAAATTGTAATACTTCAGTCATTAAGCAGGCTTGTCCAAAGATAT
bz162               GCTAATAAATTGTAATACCTCAACCATTACACAGGCTTGTCCAAAGGTAT
bz163               GCTAATAAATTGCAATACCTCAACCATTACACAGGCTTGTCCAAAAGTAT
ru131               GCTAATAAATTGTAATGTCTCAACCATTAAACAGGCTTGTCCAAAGGTGA
lbv21               GCTAATAAATTGTAATGCCTCAACCATTAAACAAGCTTGTCCAAAGGTGA
vi525               GCTAATACATTGTAATGTCTCAACCATTAAACAGGCTTGTCCCAAGGTAT
vi557               --------------------------------------------------
se9173              TCTAATAAACTGTAATACCTCAGTTATTAAACAGGCTTGTCCAAAGGTGT
se9280              TCTAATAAACTGTAATACCTCAGTCATTAAACAAGCTTGTCCAAAGGTGT
primer_ed5          --------------------------------------------------
primer_ed31         -----------------CCTCAGCCATTACACAGGCCTGTCCAAAG----
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               CCTTTGAGCCAATTCCCATACATTATTGTGCTCCAGCTGGTTTTGCGATT
br20                CCTTTGAGCCAATTCCCATACATTATTGTACCCCGGCTGGTTATGCGATT
th14                CCTTTGAGCCAATTCCCATACATTATTGTACCCCGGCTGGTTTTGCGATT
sf162               CCTTTGAACCAATTCCCATACATTATTGTGCCCCGGCTGGTTTTGCGATT
ma959               CTTTTGACCCAATTCCTATACATTATTGTACTCCAGCTGGTTATGCGATT
zm18                CTTTGGATCCAATTCCTATACATTATTGTGCTCCAGCTGGATATGCGATT
in868               CTTTTGATCCAATTCCTATACACTATTGTGCTCCAGCTGGTTTTGCGATT
ug21                CCTTTGAGCCAATTCCCATACATTATTGTGCCCCAGCTGGATTTGCAATT
ug46                --------------------------------------------------
th22                CCTTTGATCCAATTCCTATACATTATTGTACTCCAGCTGGTTATGCGATT
th06                CCTTTGATCCAATTCCTATACATTATTGTGCTCCAGCTGGTTATGCGATT
bz162               CTTGGGATCCTATTCCCATACATTATTGTGCTCCAGCTGGTTATACGATT
bz163               CTTGGGGTCCAATTCCCATACATTATTGTGCTCCAGCTGGTTATGCGATT
ru131               ATTTTGACCCAATTCCCATACATTATTGTGCTCCAGCTGGTTTTGCGATT
lbv21               CTTTTGACCCAATTCCCATACATTATTGCGCTCCAGCTGGGTTTGCGATT
vi525               CTTTTGAACCAATTCCCATACATTATTGTGCTCCAGCTGGTTTTGCAATT
vi557               --------------------------------------------------
se9173              CTTTTCAACCAATTCCCATACATTATTGTGCTCCAGCTGGTTTTGCGATT
se9280              CTTTTCAACCAATTCCCATACATTATTGTGCCCCAGCTGGTTTTGCGATT
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               CTAAAGTGTAATGATGCAGAGTTCAATGGAACAGGGCCATGCAAGAATGT
br20                CTAAAGTGTAATGGTAAGAAGTTCAATGGAACAGGACCCTGTACAAATGT
th14                CTACAGTGTAACAATAAAAAGTTCAATGGAACAGGACCATGTACAAATGT
sf162               CTAAAGTGTAATGATAAGAAGTTCAATGGATCAGGACCATGTACAAATGT
ma959               CTAAAGTGTAATAATAAGACATTCAATGGGACAGGACCATGCCATAATGT
zm18                CTAAAGTGTAATAATAAGACATTCAATGGGACAGGACCATGCCATAATGT
in868               ATAAAGTGTAATAATAAGACATTCAATGGGACAGGACCATGCAGTAATGT
ug21                CTAAAATGTAATGATAAAAAGTTCAATGGGACAGGTCCATGTAAAAATGT
ug46                --------------------------------------------------
th22                TTAAAGTGTAATGACAAGAATTTTACTGGGACAGGGCCATGTAAAAATGT
th06                TTAAAGTGTAATGATAAAAACTTCAATGGGACAGGGCCATGTAAAAATGT
bz162               CTAAAGTGTAAGGATAAAAAGTTCAATGGGACAGGGCCATGCGAGAATGT
bz163               TTAAAGTGTAATGATAAAAAGTTCAATGGGACAGGGCCATGCAAGAATGT
ru131               TTAAAGTGTAGGGATAAGAATTTCACTGGAACAGGACCATGTAAAAATGT
lbv21               CTAACATGTAGGGATAAGAAGTTCAATGGAACAGGACCGTGTAAAAATGT
vi525               TTAAAGTGTAGGGAGAAGAATTTCACTGGAACAGGACTATGTAAAAATGT
vi557               --------------------------------------------------
se9173              CTAAAATGTAATAATAAGACTTTCAATGGAACAGGACCATGCAAAAATGT
se9280              CTAAAATGTAATGATAAGAATTTCAATGGAACAGGATCATGCAAAAATGT
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               CAGCACAGTACAATGCACACATGGAATCAGGCCAGTA-ATATCAACCCAA
br20                CAGCACAGTACAATGTACACATGGAATTAAGCCAGTA-GTGTCCACTCAA
th14                CAGTACAGTACAATGTACACATGGAATTAGGCCAGTA-GTATCAACTCAA
sf162               CAGCACAGTACAATGTACACATGGAATTAGGCCAGTA-GTGTCAACTCAA
ma959               CAGCACAGTACAGTGTACACATGGAATTAAGCCAGTG-GTATCAACTCAA
zm18                CAGCACAGTACAATGTACACACGGAATCAAGCCAGTG-GTATCAACTCAA
in868               CAGCACAGTACAATGTACACATGGAATTAAGCCAGTG-GTATCAACTCAA
ug21                CAGCACAGTACAGTGTACACATGGGATTAAGCCAGTA-GTGTCAACTCAA
ug46                --------------------------------------------------
th22                CAGCTCAGTACAATGCACACATGGAATTAAGCCAGTG-GTATCAACTCAA
th06                CAGCTCAGTACAATGCACACATGGAATTAAGCCAGTG-GTATCAACTCAA
bz162               CAGCACAGTACAATGTACACATGGAATTAAACCAGTG-GTATCAACTCAA
bz163               CGC-ACAGTACAGTGTACACATGGAATTAAACCAGTG-GTATCAACTCAA
ru131               CAGTACAGTACAATGTACACATGGAATTAAGCCAGTG-GTATCAACTCAA
lbv21               CAGTACAGTGCAATGTACACATGGAATTAAGCCAGTG-GTATCAACTCAA
vi525               CAGTACAGTACAATGTACACATGGAATTAAGCCAGTG-GTATCAACTCAA
vi557               --------------------------------------------------
se9173              CAGTACAGTACAATGTACACACGGAATTAAGCCAGTG-GTATCAACTCAA
se9280              CAGTACAGTACAATGTACACACGGAATTAAGCCAGTG-GTATCAACTCAA
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          -----------------------------------TGTAAAACGACGGCC
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               CTGCTGTTAAATGGCAGTCTAGCAGAAGGAAGGGTAAAGATTAGATCTGA
br20                CTGCTGTTAAATGGCAGTCTAGCAGAAGAAGATATAGTAATTAGATCTGA
th14                CTGCTGTTAAATGGCAGTTTAGCAGAAGAAGAGGTAGTAATTAGATCTAG
sf162               TTGCTGTTAAATGGCAGTCTAGCAGAAGAAGGGGTAGTAATTAGATCTGA
ma959               CTACTGTTAAATGGTAGCCTAGCAGAAGGAGAAATAATAATTAGATCTGA
zm18                CTACTGTTAAATGGTAGCCTAGCAGAAGAAGAGATAATAATTAGGTCTGA
in868               CTACTGTTAGATGGTAGCCTAGCAGAAGAAGGGATAATAATTAGATCTGA
ug21                CTGTTGTTGAATGGCAGTCTAGCAGAAGAAGAGATAATAATTAGATCTGA
ug46                ---------------------------------ATAATAATTAGATCTGA
th22                TTGCTGTTAAATGGCAGTCTAGCAGAAGAAGAGATAATAATCAGATCTGA
th06                TTGCTGTTAAATGGCAGTCTAGCAGAAGAAGAGATAATAATCAGATCTGA
bz162               TTGTTGTTAAATGGCAGCCTAGCAGAAGAAAGTATAGTAATCAGATCTCA
bz163               TTGTTGTTAAATGGCAGCCTAGCAGAAGAAGATATAATAATCAGATCTCA
ru131               TTATTGCTGAATGGCAGCTTAGCAGAAGAAGAAATAATAGTTAGATCTGA
lbv21               TTACTGCTGAATGGCAGTTTAGCAGAAGAAGAAATAATGATTAGATCTGA
vi525               CTACTGCTGAATGGCAGTTTAGCAGAAAAAGAAATTATAATTAGATCTGA
vi557               ---------------------------AACCATGTAATAATTAGATCTAA
se9173              CTACTGCTAAATGGCAGTGTAGCAGAAGGAGACATAATAATTAGATCTGA
se9280              CTACTGCTAAATGGCAGTATAGCAGAAGGAGACATAATAATTAGATCTGA
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          AGTCTGTTAAATGGCAGTCTAGC---------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               AAATATCACAAACAATGCCAAAACCATAATAGTACAACTTAACAAGACTG
br20                AAATCTCACAAACAATGCTAAAACCATAATAGTGCAGCTAAAGGACCCTG
th14                CAATTTCACGGACAATGCTAGAGTCATAATAGTACAGCTGAATGAATCTG
sf162               AAATTTCACAGACAATGCTAAAACTATAATAGTACAGCTGAAGGAATCTG
ma959               AAATCTGACAAACAATGTCAAAACAATAATAGTGCACCTTAATGAATCTG
zm18                AAATCTAACAAACAATGCCAAAACAATAATAGTACATCTTAATGAATCTG
in868               AAATCTGACAAACAATGCCAAAATAATAATAGTACATCTTGATCAACCTG
ug21                AAATCTCACAAATAATGCTAAAATTATAATAGTACATCTTAATGAGTCTG
ug46                AAATCTCACAAATAATGCTAAAACCATAATAGTACAGCTTAATGAGTCTG
th22                AAATCTCACAAACAATGCCAAAACCATAATAGTGCACCTTAATAAATCTG
th06                AAATCTCACAAACAATGCCAAAACCATAATAGTGTACCTTAATAAATCTG
bz162               AAATATCTCAGATAATGCAAAAATCATAATAGTACACCTTAATGAATCTG
bz163               AAATATCTCAGATAATGCAAAAACCATAATAGTACACTTTAATGAATCTG
ru131               AAACTTCACAGACAATGCCAAAGTCATAATAGTGCAGCTTAATAAATCTG
lbv21               AAACTTCACAAACAATGCCAAAAACATAATAGTGCAATTTAATAAATCTA
vi525               AAACTTCACAAACAATGCCAAAGTCATAATAGTGCAGCTTAATGAAACTG
vi557               AAATATCACAGACAATACCAAAAACATAATAGTACAGCTTAAAAGTCCTG
se9173              AAATATCTCAGACAATGCTAAAAACATAATAGTACAACTTAATGACACTG
se9280              AAACATCTCAGACAATGCCAAAAACATAATAGTACAACTTAATAAAACTG
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               TAGAAATTAATTGTACCAGACCTAACAACAATACAAGAAAAAGTGTACGT
br20                TAGACATTAATTGTACAAGACCCAACAACAATACAAGAAAGAGTATACAT
th14                TAGAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATACAT
sf162               TAGAAATTAATTGTACAAGACCTAACAATAATACAAGAAAAAGTATAACT
ma959               TAGAAATTGTGTGTACAAGACCCAACAATAATACAAGAAGAAGTATAAGA
zm18                TAGAAATTGTGTGTACAAGACCCAGCAATAATACAAGAAAAAGTATAAGG
in868               TAGAAATTGTGCGTACAAGACCAAACAATAATACAAGAAAAAGTATAAGG
ug21                TACCAATTAATTGCACAAGGCCCTACGATAAGGTAAGCTACAGGACACCT
ug46                TACCAATTAATTGCTCAAGGCCCTACGAAAATAAAAGACGACGTACACCT
th22                TAGAAATCAATTGTACCAGACCCTCCAACAATACAAGAACAAGTATAACT
th06                TAGAAATCAATTGTACCAGACCCTCCAACAATACAAGAACAAGTATAACT
bz162               TACAAATTAATTGTACAAGACCCAACAACAATACAAGAAAAAGTATACAT
bz163               TACAGATTAATTGTACAAGACCCAACAACAATACAAGAAAAGGTATACAT
ru131               TAGAAATTACATGTACCAGACCTAATAACAATACAAGAAAAAGTATAACT
lbv21               TAGATATTGTGTGTACCAGACCCAACAACAATACAAGAAAAAGTATACAC
vi525               TAGAAATTAATTGTACCAGACCCAACAACAATACAAGAAAAAGTATAAAA
vi557               TACCAATTAACTGTACCAGACCCAACAATAATACGAGAAAAAGTATAAGT
se9173              TAGAAATTGTGTGTACCAGACCTAATAACAATACAAGAAAAGGTATACAC
se9280              TAGAAATTGTGTGTTACAGACCTAATAACAATACAAGGAAAGGTATACAC
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               ATAGGACCAGGACAAGCATTCTATGCAACAGGTGACATAATAGGGGATAT
br20                ATAGGACCAGGGAGAGCATTTTATGCAACAGGAGACATAATAGGAGATAT
th14                CTAGGACCAGGGCGAGCATGGTATACAACAGGACAAATAATAGGAGATAT
sf162               ATAGGACCGGGGAGAGCATTTTATGCAACAGGAGACATAATAGGAGATAT
ma959               ATAGGACCAGGACAAGTATTCTATGCAAATAATGACATAATAGGAGACAT
zm18                ATAGGACCAGGACAAGCATTCTATGCAACAGGTGGCATAATAGGAAACAT
in868               ATAGGACCAGGACAAACATTCTATGCAACAGGAGACATAATAGGAGACAT
ug21                ATAGGAGTAGGGCGAGCATCCTATACAACAAG---AATAAAAGGAGATAT
ug46                ATAGGACTAGGGCAAGCGTACTATACAACAAA---ATTAAAAGGATATAT
th22                ATAGGACCAGGACAAGTATTCTATAGAACAGGAGACATAATAGGAGATAT
th06                ATAGGACCAGGACAAGTATTCTATAGAACAGGAGACATAATAGGAGATAT
bz162               ATAGGACCAGGACGGGCACTTTATGCAACAGGAGACATAATAGGAGACAT
bz163               ATAGGACCAGGACGAGCATTTTATGCAACAGGAGACATAATAGGAGACAT
ru131               TTTGCACCAGGACAAGCGCTCTATGCAACAGGTGAAATAATAGGAGATAT
lbv21               ATAGGACCTGGACAAGCATTATATGCAACAGGCGCCATAATAGGCGATAT
vi525               TTCGGAACAGGACGAGTGTTATATGCAACAGGTGCCATAATAGGAAATAT
vi557               ATAGGGCCAGGACAAGCATTCTATGCAACAGGTGACATCATAGGAGATAT
se9173              ATGGGACCAGGACAAGTGCTCTACGCAACAGGGGAAATAATAGGAGATAT
se9280              ATGGGACCAGGACAAGTGCTCTACGCAACAGGAGAAATAATAGGAAATAT
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               AAGACAAGCATATTGTAATGTCAGTAGAGCAGACTGGAATAAAACTTTAC
br20                AAGACAAGCACATTGTAACCTTAGCAGAGCACAATGGAATGACACTTTAA
th14                AAGACAAGCACATTGTAACCTTAGTAGCACAAAATGGAATAACACTTTAA
sf162               AAGACAAGCACATTGTAACATTAGTGGAGAAAAATGGAATAACACTTTAA
ma959               AAGACAAGCACATTGCAACATTAGTAAGGATGTCTGGAACAGTACTTTAC
zm18                AAGACAAGCACATTGTAACATTAGTAAAGAGAACTGGAATAAAACTTTAC
in868               AAGACGAGCATATTGTAACATTAGTGAAAACAAATGGAATGAAACTTTAC
ug21                AAGACAAGCACATTGTAACATTAGTGGAGAAAAATGGAATAAAACTTTAC
ug46                AAGACCAGCACATTGTAATATTAGTGGAGCAGAATGGAATAAAACTTTAC
th22                AAGAAGAGCATATTGTGAGATTAATGGAACAAAATGGAATAAAGCTTTAA
th06                AAGACAAGCATATTGTGAGATTAATGAAACAAAATGGAATGAAACTTTAA
bz162               CAGAAAGGCACATTGTAACGTTAGTAGAACACAATGGAATAAGAAGTTAG
bz163               CAGAAAGGCCCATTGTAATGTTAGTGGAACACAATGGAATAAAACATTAG
ru131               AAGACAAGCACATTGTAATGTTAGTAGAAAAGATTGGAATGAGATGTTGC
lbv21               AAGACAAGCACATTGTAATGTTAGTGAAACAGACTGGAGAGACATGTTAC
vi525               AAGACAAGCACATTGTAATGTTAGTACAGAAAAATGGGGTAAGATGTTAC
vi557               AAGAAAAGCATATTGTAATATTACTAGGGAAGATTGGAAAAGGACTTTAC
se9173              AAGGAAAGCATATTGTAACATTAGTAGAAAAGATTGGAATAACACTTTAC
se9280              AAGAGAAACACATTGTAACATTAGTGAAAGAGATTGGAGTAACACTTTAC
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               AAGGGGTAGCCAACCAATTAAAAAGTTACTTTAGTAACAAAACAATAATC
br20                GCAAGATAGTTACAAAATTAAGAGAACAATTTGAGAATAAAACAATAAAG
th14                GACAGATAACTGAAAAATTAAGAGAACAATTTGGGAACAAAACAATAGTC
sf162               AACAGATAGTTACAAAATTACAAGCACAATTTGGGAATAAAACAATAGTC
ma959               AAAAGGTAGGTAAAAAATTAAAAGAACACTTCCCTAATAAAACAATAACA
zm18                AAAAGGTAGGAAAAAAATTAGCAGAGCACTTCCCTAATAAAACAATAAAA
in868               AAAGGGTGAGTAAAAAATTAGCAGAATACTTCCCTAATAAAACAATACAA
ug21                AACAGGTAGCTGCGAAATTAAGAGACCTTCTTAACCAAACAGCAATAATT
ug46                AACAGGTAGCTAAAAAATTAGGAGACCTTTTCAACCAGACAACAATAATT
th22                AACAGGTAACTGAAAAATTAAAAGAGCACTTTAAT---AAGACAATAATC
th06                AACAGGTAACTAAAAAATTAAAAGAGCACTTTAATAATAAGACAATAATC
bz162               ACCAGGTAAAGGCAGAGTTAAAGTCTCATTTCCCTAATGCAACAATACAA
bz163               AACAAGTAAGGGCAAAGTTAAAGTCTCATTTTCCTAATGCAACAATAAAA
ru131               AGAATGTGACTACAAAACTAAAAGGAATCTTTAACAACACAAACATAACA
lbv21               AGAAGGTAAAAGCACAACTACAAGGAATCTACAACAAAAGC---ATAACC
vi525               AGAATGTAGCTGCAAAACTACAAAAAGAATTTAATAAA---AACATAACC
vi557               ATGAGGTAGTTCAGCAATTAAGAGAACACTTTAATAATCAAACAATAATT
se9173              GTAGAGTAGCTAAAAAACTAAGAGAACACTTTAATAAAACA---ATAGAC
se9280              GTAGAGTAGCTACAAAACTAAGAGAACACTTTAATAAAACA---ATAAAC
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               TTTGCTAGCTCCTCAGGAGGGGATTTAGAAATTACAACACATAGTTTTAA
br20                TTTCAGCCACCCTCAGGAGGGGATCCAGAAATTGTATTTCACAGTTTTAA
th14                TTTAATCAATCCTCAGGAGGGGACCCAGAGATTGTAATGCACAGTTTTAA
sf162               TTTAAGCAATCCTCAGGAGGGGACCCAGAAATTGTAATGCACAGTTTTAA
ma959               TTTGAACCACACTCAGGAGGAGATCTAGAAATTACAACACATAGTTTTAA
zm18                TTTGACCAACACTCAGGAGGGGACCTAGAAATTACAACACATAGCTTTAA
in868               TTTGCATCATCCTCAGGAGGGGACCTAGAAATTACAACACATAGCTTTAA
ug21                TTTAAACCATCCTCGGGAGGGGACCCAGAAATTACAACACACAGCTTTAA
ug46                TTTCAACCA-----------------------------------------
th22                TTTCAACCACCCTCAGGAGGAGATCTAGAAATTACAATGCATCATTTTAA
th06                TTTCACCCACCCTCAGGAGGAGATCTAGAAATTATAACGCATCATTTTAA
bz162               TTTAACTCATCCTCAGGAGGGGACCTAGAAGTTACAATGCATCATTTTAT
bz163               TTTAATTCATCCTCAGGAGGGGACCTAGAAATTACAATGCATAGTTTTAA
ru131               TATAACTCAGCTGCAGGAGGAGATCTAGAAATTACAACACATAGTTTTAA
lbv21               TTTAACTCATCCTCAGGAGGGGACCTAGAAATTACAGCACATAGTTTTAA
vi525               TTTAACTCATCCTCAGGAGGGGACCTAGAAATTACAACACATAGTTTTAA
vi557               TTCGAACCATCCTCAGGAGGGGACATGGAAATTACAATGCATACTTTTAA
se9173              TTTACATCACCCTCAGGAGGGGACATAGAAATTACAACACATAGTTTTAA
se9280              TTTACATCACCCTCAGGAGGGGATATAGAAATTGTGACACATAGTTTTAA
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               TTGTGGAGGAGAATTTTTTTATTGTGATACATCAGGCCTGTTTAATAGCA
br20                TTGTGGAGGGGAATTTTTCTACTGTAATACAACACAACTGTTTAATAGTA
th14                TTGTGGAGGGGAATTTTTCTACTGTAATACAACACAACTGTTTAATAGTA
sf162               TTGTGGAGGGGAATTTTTCTACTGTAATTCAACACAGCTTTTTAATAGTA
ma959               TTGTAGAGGAGAATTTTTCTATTGCAATACATCAGGGCTGTTTAATAGTA
zm18                TTGTAGAGGAGAATTTTTCTATTGCAATACATCAAACCTGTTTAATAGTA
in868               TTGTAGAGGAGAATTTTTCTATTGCAATACATCAAACCTGTTTAATGGTA
ug21                TTGTGGAGGGGAATTTTTCTACTGCAATACATCAGGACTGTTTAACAATA
ug46                --------------------------------------------------
th22                TTGTAGAGGGGAATTTTTCTATTGCAATACAACACGACTGTTTAGTAATA
th06                TTGTAGAGGGGAATTTTTCTATTGCAATACACCAAAATTGTTTAATGGAA
bz162               TTGTAAAGGAGAATTTTTCTACTGCGATACATCAAGACTGTTCAGTAACA
bz163               TTGTAGAGGAGAATTTTTCTACTGCAATACATCAGGACTGTT--------
ru131               TTGTAGAGGAGAATTTTTCTATTGCAATACATCAGGACTGTTTAATAATA
lbv21               TTGTAGAGGAGAATTTTTCTATTGTAATACATCAGGACTGTTTAATAGTA
vi525               TTGTAGAGGAGAATTTTTTTATTGTAAGACATCAGAGCTGTTTAATAGTA
vi557               TTGTAGAGGAGAATTTTTCTATTGCAATACATCAAAATTGTTTAACAGTA
se9173              TTGTGGAGGAGAATTTTTCTATTGTAATACATCAACACTGTTCAATAGTA
se9280              TTGTGGAGGAGAATTTTTATATTGTAATACATCAAAACTGTTCAATAGTA
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  -----GAGGGGAATTTTTCTACTGTAA-----------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               CATGGGGGGGAAATAGCACTGACAGCATACAGGAGTCAAATAGCACAGAG
br20                CCTGGACAAATAACAC---TGAAGGGACAAGTAACACTACAGGGAATGAC
th14                CTTGGAATGATACTAG---TACTTGG---AATAATAATACAGGAAATGGC
sf162               CTTGGA---ATAATAC---TATAGGGCCAAATAACACT------AATGGA
ma959               ---------ACTTTAA---TGATACAGAAGGTAATTCA------ACTTTA
zm18                CATATA---AGCCTAA---TGATACAAATAGTACATATAATCCTAATGAT
in868               ------CATACATGAA---TGGTACAAAAAGTAATTCA------AGCTCA
ug21                GTGTATGGACATCAAA---TAGT---ACGATAGGGGCA------AATGGG
ug46                --------------------------------------------------
th22                CTTGCATAAAAAATGA---AACCATGGCGGGG---TGT------AACGGC
th06                CCATGGAGGGGCCTAA---TAACACGGAGGGGCCTAAT------AACACT
bz162               C---------------------AGAATCCAATGGCACAGAATCCAATGGC
bz163               ----------------------------TAACGACACAGGATCCAATGGC
ru131               G---------TGAGAA---TAACTCCACTATCGAGAAT------AACTCC
lbv21               GTATATTATCTAACAA---TAATGCACCATCTAATAGC------AGTAAG
vi525               CTTGGTTGTTTAATAGTACTG---GGCTGGTTAATAGTACTTGGAATGAT
vi557               CTTGGG---TTAACAG---TACATCAAATGATATCTCAAATGGCACAACA
se9173              GTTGGGATGAGAATAACATTAAGGACACAAATAGTACAAATGACAACACA
se9280              GTTGGGATAAGAATAGCATTGAAGCTACAAATGACACAAGT---NACGCA
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               TCAAATGACACTATAATTCTCCCATGCAGAATAAAGCAAATTATAAATAT
br20                AC---------AATCACACTCCCATGTAGAATAAAACAAATTGTAAACAT
th14                AC---------TATCACGCTCCCTTGCAGAATAAAACAAATTGTAAACAT
sf162               AC---------TATCACACTCCCATGCAGAATAAAACAAATTATAAACAG
ma959               AG---------CATAACCCTCCCATGCAGAATAAAACAAATTATAAACAT
zm18                AC---------AATCACACTCCCATGCAGAATAAAACAAATTATAAACAT
in868               AC---------CATCACAATCCTATGCAGAATAAAACAAGTTGTAAACAT
ug21                AC---------AATCACACTCCCATGCAGAATAAAACAAATTATAAATAT
ug46                --------------------------------------------------
th22                AC---------TATCATACTTCCATGCAAGATAAAGCAAATTATAAACAT
th06                AC---------TATCATACTTCCATGCAAGATAAAGCAAATTGTAAACAT
bz162               AC---------TATCACTCTCCCATGTCGAATAAAACAAATGGTAAACAT
bz163               AC---------TATCACTCTCCTATGTCGAATAAAACAAATGGTAAACAT
ru131               AC---------TATCACACTCCCATGTAGGATAAAACAAATTATAATAAT
lbv21               AA---------TATCACACTCCCATGTAGAATAAAACAAATTGTAAGAAT
vi525               ACACGAAAAGATATTACACTGCAATGCAAAATAAAACAAATTGTGAGAAT
vi557               AT---------AATTGAACTGCCATGCAGAATAAAACAAATTATAAACAT
se9173              AC---------TATCACAATACCATGTAAAATAAAACAAATTGTGAGAAT
se9280              AC---------TATCACAATACCATGTAAAATAAAACAAATTGTGAGAAT
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               GTGGCAGAGAGTAGGACAAGCAATGTATGCCCCTCCCATC---CAAGGAG
br20                GTGGCAGGAAGTAGGAAAAGCAATGTATGCCCCTCCCATC---AAGGGAA
th14                GTGGCAGGAAGTAGGAAAAGCAATGTATGCCCCTCCCATC---AGAGGAC
sf162               GTGGCAGGAAGTAGGAAAAGCAATGTATGCCCCTCCCATC---AGAGGAC
ma959               GTGGCAGGAGGTAGGACGAGCAATGTATGCCCCTCCCATT---GAAGGAA
zm18                GTGGCAGGGGGTAGGACAAGCAATGTATGCCCCTCCCATT---GCAGGAA
in868               GTGGCAGGAGGTAGGACGAGCAATGTATGCCCCTCCCATT---GAAGGAA
ug21                GTGGCAGGGAGTAGGAAAAGCAATGTATACCCCTCCCATT---GAAGGAC
ug46                --------------------------------------------------
th22                GTGGCAGGGAGCAGGACAAGCAATGTATGCTCCTCCCATT---AGGGGAA
th06                GTGGCAGGGAGCAGGACAAGCAATGTATGCTCCTCCCATC---AGTGGAA
bz162               GTGGCAGGAAGTAGGACGAACAATGTATGCCGCTCCCATC---GCAGGAA
bz163               GTGGCAGGAAGTGGGACGAGCAATGTATGCCGCTCCCATC---TCAGAAA
ru131               GTGGCAGAGAGTGGGGCAAGCAATGTATGCCCCTCCCATC---GCAGGAA
lbv21               GTGGCAGAGGGTGGGACAAGCAATGTATGCCCCTCCCATT---GCAGGAA
vi525               GTGGCAGAGAGTGGGACAAGCAATGTATGCCCCTCCCATCGCAGCAGGAA
vi557               GTGGCAGAGAGTAGGACAAGCAATGTATGCCCCTCCCATC---AAAGGAA
se9173              GTGGCAAAGAACAGGACAAGCAATATATGCCCCTCCCATC---GCAGGAA
se9280              GTGGCAAAGGACAGGACAAGCAATCTATGCCCCTCCCATT---GCAGGAA
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               TAATAAAGTGTATATCAAATATTACAGGACTTCTATTAACAAGAGATGGT
br20                AAATTAAATGTTCATCAAACATTACAGGGCTACTATTGACAAGGGATGGT
th14                AAATTAGATGTTCATCAAATATTACAGGGTTGCTATTAACAAGAGATGGT
sf162               AAATTAGATGCTCATCAAATATTACAGGACTGCTATTAACAAGAGATGGT
ma959               ACATAACATGTAAATCAAATATCACAGGACTACTATTGACACGTGGTGGA
zm18                ACATAACATGTAAATCAAATATCACAGGACTACTATTGACACGGGATGGA
in868               ACATAACATGTAAATCAAATATCACAGGACTACTATTGGCACGTGATGGA
ug21                AAATCAACTGTTCATCAACTATTACAGGACTATTGTTGACAAGAGATGGT
ug46                --------------------------------------------------
th22                GAATTAATTGTGTATCAAATATTACAGGAATACTATTGACAAGGGATGGT
th06                GAATTAATTGTGTATCAAATATTACAGGAATACTATTAACAAGAGATGGT
bz162               ACATTACCTGTAACTCAAATATTACAGGTCTACTATTGACAAGAGACGGT
bz163               ACATTACCTGTAACTCGAATATTACAGGTCTACTATTGACAAGAGATGGT
ru131               ACATCACATGTATTTCAAACATTACAGGACTACTATTAACAAGAGATGGT
lbv21               ACATTACATGTAAATCAAACATTACAGGACTAATATTAACAAGAGATGGT
vi525               ACATTGAATGTAGATCAAGCATTACAGGACTAATATTAACAAGAGATGGT
vi557               AAATTACATGTGAATCAAATATTACAGGGCTAATATTGACAAGTGATCAT
se9173              ACATTACATGCAAATCAAATATTACAGGATTATTATTGACACGTGATGGA
se9280              ACATTACGTGCACATCAAATATTACAGGATTACTATTGACACGTGATGGA
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               GG---------GGA------TAATAACAGTGCA------AATGAAACCTT
br20                GG---------TAA------TAATGAGATGAAC---ACGACCGAGATCTT
th14                GG---------TAA------TAGTGAGAACAAG---ACCACCGAGACCTT
sf162               GG---------TAA------AGAGATCAGTAAC---ACCACCGAGATCTT
ma959               GG---------TCC------A---ACTAACACA---AAGACAGAGACATT
zm18                GG---------GTC------AAATGATACCACA---AACACAGAGACATT
in868               GG---------AAC------A---GAGTCAAAT---CATACAGAGATATT
ug21                GG---------TGT------GAAAAATAATAGT---CAGAACGAGACCTT
ug46                --------------------------------------------------
th22                GG---------TGC------TAATAATGCG---------AACGAGACCTT
th06                GG---------TGC------TAATAATACGAC------TAACGAGACCTT
bz162               GGGTTAGATGATGGTAGGAATACTAGTCAGACTAATCGGACTGAGATCTT
bz163               GG---------TCA------GAATAATAATCAG------ACTGAGACCTT
ru131               GG---------GAA------CAATAATAACAGCACAAATAATGAGACCTT
lbv21               GG---------GAA------TAGTACCAATGGA------AATGAGACCTT
vi525               GG---------GAA------TAATAATAATACC------AATGAGACCTT
vi557               G---------------------TTAATTCTGCAGAAAGTAATGTTACCTT
se9173              GG---------AAACA---GGAATGGCAGTGAGAATGGCACTGAGACCTT
se9280              GG---------AAACAGGGGGAATGGCAGTGAGAATGGCACTGAGACCTT
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               CAGACCTACAGGAGGAAATATGAGGGACAATTGGAGAAGTGAATTATATA
br20                CAGACCTGGAGGAGGAGATATGAGGGACAATTGGAGGAGTGAATTATATA
th14                CAGACCTGGAGGAGGAGATATGAGGGACAATTGGAGAAGTGAATTATATA
sf162               CAGACCTGGAGGTGGAGATATGAGGGACAATTGGAGAAGTGAATTATATA
ma959               CAGGCCTGGAGGAGGAGATATGAGGGACAATTGGAGAAGTGAATTATATA
zm18                CAGACCTGGAGGAGGAGATATGAGGGACAATTGGAGAAGTGAACTATATA
in868               CAGACCTGGAGGAGGAGATATGAGGAACAATTGGAGAAGTGAATTATATA
ug21                CAGACCTGGAGGGGGAGATATGAGAGACAACTGGAGAAATGAATTATACA
ug46                --------------------------------------------------
th22                CAGACCTGGAGGAGGAAATATAAAGGACAATTGGAGAAGTGAATTATATA
th06                CAGACCTGGAGGAGGAAATATAAAGGACAATTGGAGAAGTGAATTATATA
bz162               CAGACCTGGGGGAGGAAATATGAAAGACAATTGGAGAAGTGAATTATATA
bz163               CAGACCTGGGGGTGGTGATATGAAAGACTATTGGAGAAGTGTATTATATA
ru131               CAGACCTGGAGGAGGAGATATGAGGGACAATTGGAGAAGTGAATTATATA
lbv21               CAGACCTGCAGGAGGAGACATGAGGGACAATTGGAGAAGTGAATTATATA
vi525               CAGACCTGGAGGAGGAGACATGAGGGGCAATTGGAGAAGTGAATTATATA
vi557               CAGACCTGGAGGAGGAGATATGAGGGACAATTGGAGAAGTGAATTATATA
se9173              CAGACCTACAGGAGGAAATATGAAAGATAATTGGAGAAGTGAATTATATA
se9280              CAGACCTACAGGAGGAAATATGAAGGACAATTGGAGAAGTGAATTATATA
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------TGAGGGACAATTGGAGAAGTGGGTCATAGC
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               AGTATAAAGTAGTAAAAATTGAACCACTAGGAGTAGCACCCACCCCGGCA
br20                AATATAAAGTAGTAAGAATTGAACCATTAGGAATAGCACCCACCAGGGCA
th14                AATATAAAGTAGTGAAAATTGAACCATTAGGAGTAGCACCCACCAAGCCA
sf162               AATATAAAGTAGTAAAAATTGAGCCATTAGGAGTAGCACCCACCAAGGCA
ma959               AGTATAAAGTGGTAGAAATTAAGCCATTGGGAGTAGCACCCACTAAGGCA
zm18                AATATAAAGTGGTAGAAATTAAACCATTGGGCATAGCACCCACTGCGGCA
in868               AATACAAAGTGGTAGAAATTAAGCCATTGGGAGTAGTACCCACTACAGCA
ug21                AATATAAAGTAGTAAGAATTGAACCACTAGGTCTAGCACCCACCAAGGCA
ug46                --------------------------------------------------
th22                AATATAAAGTAGTACAAATTGAACCACTAGGAATAGCACCCACCAGGGCA
th06                AATATAAAGTAGTACAAATTGAACCACTAGGAATAGCACCCACCAGGGCA
bz162               AATATAAAGTAGTAGAAATTGAACCACTAGGAGTAGCACCCACCAAGGCA
bz163               AGTATAAAGTAGTAGAAATTGAACCACTAGGAGTAGCACCCACCAGGGCA
ru131               AGTATAAAGTAGTAAGAATTAAACCACTAGGAGTAGCACCCACCAGG---
lbv21               AGTATAAAGTAATAAAAATTAAACCACTAGGAGTAGCACCCACCAAGGCA
vi525               AGTATAAAGTAGTAAAAATCAAATCACTAGGAGTAGCACCCACCAGGGCA
vi557               AATATAAAGTAGTAAGAGTCGAACCACTAGGATTAGCACCCACCAGGGCA
se9173              AATATAAAGTAGTAGAGCTTGAGCCACTAGGAGTAGCACCCACCAAGGCA
se9280              AATATAAAGTAGTAGAGATTGAGCCACTAGGAGTAGCACCCACCAAGGCA
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   TGTTTCCTG-----------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               AAGAGAAGAGTGGTGCAG---AGAGAAAAAAGAGCAGTTGGA---ATAGG
br20                AAGAGAAGAGTGGTGCAA---AGAGAAAAAAGAGCAGTGGGAACATTAGG
th14                AAGAGAAGAGTGGTGCAG---AGAGAAAAAAGAGCAGTGGGAACGATAGG
sf162               AAGAGAAGAGTGGTGCAG---AGAGAAAAAAGAGCAGTG---ACGCTAGG
ma959               AAAAGGAGAGTGGTGGAG---AGAGAAAAAAGAGCAGTGGGA---ATAGG
zm18                AAAAGGAGAGTGGTGGAGACGAGAGAAAAAAGAGCAGTGGGA---ATAGG
in868               AAAAGGAGAGTGGTGGAG---AGAGAAAAAAGAGCAGTGGGA---ATGGG
ug21                AGGAGAAGAGTGGTGGAA---AGAGAAAAAAGAGCAATAGGA---CTGGG
ug46                --------------------------------------------------
th22                AAGAGAAGAGTGGTGGAG---AGAGAAAAAAGAGCAGTGGGA---ATAGG
th06                AAGAGAAGAGTGGTGGAG---AGAGAAAAAAGAGCAGTGGGA---ATAGG
bz162               AAAAGACAAGTGGTGAAG---AGAGAAAAAAGAGCAGTGGGA---ATAGG
bz163               AAAAGACAAGTGGTGAGG---AGAGAAAAAAGAGCAGTGGGA---ATGGG
ru131               --------------------------------------------------
lbv21               AGGAGAAGAGTGGTGGGG---AGAGAAAAAAGAGCAATTACT------GG
vi525               AGGAGAAGAGTGGTGGAG---AGAGAAAAAAGAGCAATTGGA---CTGGG
vi557               AGGAGAAGAGTGGTGGAA---AGAGAAAAAAGAGCTGTGGGA---CTGGA
se9173              AAAAGAAGAGTGGTGGAG---AGAGAAAAAAGAGCAGTGGGA---ATAGG
se9280              AAAAGAAGAGTGGTGGAG---AGAGAAAAAAGAGCAGTGGGA---ATAGG
primer_ed5          --------------------------------------------------
primer_ed31         --------------------------------------------------
primer_es7          --------------------------------------------------
primer_ed33REVCOMP  --------------------------------------------------
primer_es8REVCOMP   --------------------------------------------------
primer_ed12REVCOMP  --------------------------------------------------
                                                                     

sf170               AGCTGTATTCATTGGGTTCTTAGGAGCAGCCGGAAGCACT
br20                AGCTATGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACT
th14                AGCTATGTTCCTTGGGTTCTTGGGAACAGCAGGAAGCACT
sf162               AGCTATGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACT
ma959               AGCTGTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACT
zm18                AGCTGTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACT
in868               AGCTGTGTTCCTTGGGTTCTTGGGAGCAGCAGGAACCACT
ug21                AGCTTTGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCACG
ug46                ----------------------------------------
th22                AGCTATGATCTTTGGGTTCTTAGGAGCAGCAGGAAGCACT
th06                AGCTATGATCTTTGGGTTCTTAGGAGCAGCAGGAAGCACT
bz162               AGCTATGTTCCTTGGGTTCTTGGGAGCAGCAGGAAGCTCT
bz163               AGCTTTGTTCCTTGGGTTCTTGAGCGCGGCAGGAAGCACT
ru131               ----------------------------------------
lbv21               AGCTGTCTTCCTTGGGTTCTTAGGAGCAGCTGGGAGCACT
vi525               AGCTGTCTTCCTTGGGTTCTTAGGAGCAGCAGGAAGCACT
vi557               AGCTGTTTTCCTTGGGTTCTTGGGAGCAGCAGGGAGCACT
se9173              AGCTGTGTTCCTTGGGTTCTTGGGAACAGCAGGAAGCACT
se9280              AGCTGTGTTCCTTGGGTTCTTGGGAACAGCAGGAAGTACT
primer_ed5          ----------------------------------------
primer_ed31         ----------------------------------------
primer_es7          ----------------------------------------
primer_ed33REVCOMP  ----------------------------------------
primer_es8REVCOMP   ----------------------------------------
primer_ed12REVCOMP  ----------CTTGGGTTCTTGGGAGCAGCAGGAAGCACT