SOP for TAE Buffer

 

University of Washington Department of Microbiology

Standard Operating Procedures for Chemicals or Processes

#1   Process

(if applicable)

Gel Electrophoresis

#2  Chemicals

1,3-Propanediol, 2-Amino-2-Hydroxymethyl , Tris Base, Glacial Acetic Acid, Disodium Edta Dihydrate

#3   Personal Protective                        Equipment (PPE)

Nitro gloves, Goggles, Lab coat

#4   Environmental /

Ventilation Controls

N/A

#5  Special Handling Procedures & Storage Requirements

Store in cool dry place.

Contain Buffer in carboy.

 

#6  Spill and Accident                         Procedures

Wear NIOSH/MSHA approved respirator, goggles, rubber boots, and heavy rubber gloves. Absorb on sand or vermiculite and place in closed container. Use absorbent napkin.

#7  Waste Disposal

Filter it through Ethidium Bromide as 1x soln.

Pour down drain and record in sewage log.

#8  Special Precautions                       for Animal Use

(if applicable)

N/A

Particularly hazardous

substance involved?

        YES:

Blocks #9 to #11 are Mandatory

   X   NO:

Blocks #9 to #11 are Optional.

#9  Approval Required

N/A

#10 Decontamination

N/A

#11 Designated Area

All parts of Lab

Name:                                                                                 Title:

Signature:                                                                                            Date:                                    

Environmental Health and Safety, Box 354400                                               

 

 

 

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