Check PCR products on 1% Agarose gel (2-3 µl PCR product in 15 µl total loading volume)

 

For gels with 42 tooth combs:

 

M A1 B1 A2 B2 � A11 B11 A12 B12 M

M C1 D1 C2 D2 � C11 D11 C12 D12 M

M E1 F1 E2 F2 � E11 F11 E12 F12 M

M G1 H1 G2 H2 � G11 H11 G12 H12 M

 

For gels with 50 tooth combs:

 

M A1 B1 A2 B2 � A11 B11 A12 B12 M E1 F1 E2 F2 � E11 F11 E12 F12

M C1 D1 C2 D2 � C11 D11 C12 D12 M G1 H1 G2 H2 � G11 H11 G12 H12

 

 

Marker is 1 kb ladder (GIBCO-BRL).

Run approximately 2 hours (bromophenol blue marker 2/3 down lane).

Department of Microbiology
School of Medicine
University of Washington
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