Supplementary Material:
6952 6962 6972 6982 6991 6996 7006 7016 7026
. . . . . . . . .
BK28 aataaaagtg agacagatag atggggattg acaaaatcat caacaacaat aacaacagca gcaccaacat cagcaccagt atcagaaaaa [90]
predicted BK28 .......... .......... .......... .......... .........c .......... .......... .......... .......... [90]
BK28 N K S E T D R W G L T K S S T T I T T A A P T S A P V S E K [30]
predicted BK28 . . . . . . . . . . . . . . . . T . . . . . . . . . . . . . [30]
7036 7046 7056 7066 7076 7086 7096 7106 7116
. . . . . . . . .
BK28 atagacatgg tcaatgagac tagttcttgt atagctcaga ataattgcac aggcttggaa caagagcaaa tgataagctg taaattcacc [180]
predicted BK28 .......... .......... .......... .......... .......... .......... .......... .......... ........a. [180]
BK28 I D M V N E T S S C I A Q N N C T G L E Q E Q M I S C K F T [60]
predicted BK28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . N [60]
7126 7136 7146 7156 7166 7176 7186 7196 7206
. . . . . . . . .
BK28 atgacagggt taaaaagaga caagacaaag gagtacaatg aaacttggta ctctacagat ttggtttgtg aacaagggaa tagcactgat [270]
predicted BK28 .......... .......... .......... .......... .......... .......... .......... .......... .......... [270]
BK28 M T G L K R D K T K E Y N E T W Y S T D L V C E Q G N S T D [90]
predicted BK28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [90]
7216 7226 7236 7246 7256 7266 7276 7286 7296
. . . . . . . . .
BK28 aatgaaagca gatgctacat gaatcactgt aacacttctg ttatccaaga atcttgtgac aaacattatt gggatactat tagatttagg [360]
predicted BK28 .......... .......... .......... .......... .......... .......... .......... .......... .......... [360]
BK28 N E S R C Y M N H C N T S V I Q E S C D K H Y W D T I R F R [120]
predicted BK28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [120]
7306 7316 7326 7336 7346 7356 7366 7376 7386
. . . . . . . . .
BK28 tattgtgcac ctccaggtta tgctttgctt agatgtaatg acacaaatta ttcaggcttt atgcctaaat gttctaaggt ggtggtctct [450]
predicted BK28 .......... .......... .......... .......... .......... .......... .......... .......... .......... [450]
BK28 Y C A P P G Y A L L R C N D T N Y S G F M P K C S K V V V S [150]
predicted BK28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [150]
7396 7406 7416 7426 7436 7446 7456 7466 7476
. . . . . . . . .
BK28 tcatgcacaa ggatgatgga gacacagact tctacttggt ttggctttaa tggaactaga gcagaaaata gaacttatat ttactggcat [540]
predicted BK28 .......... .......... .......... .......... .......... .......... .......... .......... .......... [540]
BK28 S C T R M M E T Q T S T W F G F N G T R A E N R T Y I Y W H [180]
predicted BK28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [180]
7486 7496 7506 7516 7526 7536 7546 7556 7566
. . . . . . . . .
BK28 ggtagggata ataggactat aattagttta aataagtatt ataatctaac aatgaaatgt agaagaccag gaaataagac agttttacca [630]
predicted BK28 .......... .......... .......... .......... .......... .......... .......... .......... .......... [630]
BK28 G R D N R T I I S L N K Y Y N L T M K C R R P G N K T V L P [210]
predicted BK28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [210]
7576 7586 7596 7606 7616 7626 7636 7646 7656
. . . . . . . . .
BK28 gtcaccatta tgtctggatt ggttttccac tcacaaccaa tcaatgatag gccaaagcag gcatggtgtt ggtttggagg aaaatggaag [720]
predicted BK28 .......... .......... .......... .......... .......... .......... .......... .......... .......... [720]
BK28 V T I M S G L V F H S Q P I N D R P K Q A W C W F G G K W K [240]
predicted BK28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [240]
7666 7676 7686 7696 7706 7716 7726 7736 7746
. . . . . . . . .
BK28 gatgcaataa aagaggtgaa acagaccatt gtcaaacatc ccaggtatac tggaactaac aatactgata aaatcaattt aacggctcct [810]
predicted BK28 .......... .......... .......... .......... .......... .......... .......... .......... .......... [810]
BK28 D A I K E V K Q T I V K H P R Y T G T N N T D K I N L T A P [270]
predicted BK28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [270]
7756 7766 7776 7786 7796 7806 7816 7826 7836
. . . . . . . . .
BK28 ggaggaggag atccggaagt taccttcatg tggacaaatt gcagaggaga gttcctctac tgtaaaatga attggtttct aaattgggta [900]
predicted BK28 .......... .......... .......... .......... .......... .......... .......... .......... .......... [900]
BK28 G G G D P E V T F M W T N C R G E F L Y C K M N W F L N W V [300]
predicted BK28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [300]
7846 7856 7866 7876 7886 7896 7906 7916 7926
. . . . . . . . .
BK28 gaggataggg atgtaactac ccagaggcca aaggaacggc atagaaggaa ttacgtgccg tgtcatatta gacaaataat caacacttgg [990]
predicted BK28 .......... .......... .......... .......... .......... .......... .......... .......... .......... [990]
BK28 E D R D V T T Q R P K E R H R R N Y V P C H I R Q I I N T W [330]
predicted BK28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [330]
7936 7946 7956 7966 7976 7986 7996 8006 8016
. . . . . . . . .
BK28 cataaagtag gcaaaaatgt ttatttgcct ccaagagagg gagacctcac gtgtaactcc acagtgacca gtctcatagc aaacatagat [1080]
predicted BK28 .......... .......... .......... .......... .......... .......... .......... .......... .......... [1080]
BK28 H K V G K N V Y L P P R E G D L T C N S T V T S L I A N I D [360]
predicted BK28 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [360]
8026 8036 8046 8056 8064
. . . . .
BK28 tggactgatg gaaaccaaac tagtatcacc atgagtgcag aggtggca [1128]
predicted BK28 .......... .......... ..a....... .......... ........ [1128]
BK28 W T D G N Q T S I T M S A E V A [376]
predicted BK28 . . . . . . . N . . . . . . . . [376]
FIG 1. Alignment of SIVBK28 (Env region) and deduced ancestor:
Alignment of SIVmacBK28 nucleotide and amino acid sequences to the
predicted BK28 nucleotide and inferred amino acid sequences. Positions
where the predictions matched the known sequences are indicated by dots
(.); numbers above the alignment indicate positions relative to the
sequence of SIVmac239 (GenBank accession number M33262).