Supplementary Material:
6234 6237.7 6245 6255 6265 6287 6297 6307 6317
. . . . . . . . .
AN1-EnvB atgagagtga aggggatcag gaagaattat cagcacttgt ggagatgggg caccatgctc cttgggatgt tgatgatctg tagtgctgca [90]
M R V K G I R K N Y Q H L W R W G T M L L G M L M I C S A A [30]
6327 6337 6347 6357 6367 6377 6387 6397 6407
. . . . . . . . .
AN1-EnvB gaaaaattgt gggtcacagt ctattatggg gtacctgtgt ggaaagaagc aaccaccact ctattttgtg catcagatgc taaagcatat [180]
E K L W V T V Y Y G V P V W K E A T T T L F C A S D A K A Y [60]
6417 6427 6437 6447 6457 6467 6477 6487 6497
. . . . . . . . .
AN1-EnvB gatacagagg tacataatgt ttgggccaca catgcctgtg tacccacaga ccccaaccca caagaagtag tattggaaaa tgtgacagaa [270]
D T E V H N V W A T H A C V P T D P N P Q E V V L E N V T E [90]
6507 6517 6527 6537 6547 6557 6567 6577 6587
. . . . . . . . .
AN1-EnvB aattttaaca tgtggaaaaa taacatggta gaacagatgc atgaggatat aatcagttta tgggatcaaa gcctaaagcc atgtgtaaaa [360]
N F N M W K N N M V E Q M H E D I I S L W D Q S L K P C V K [120]
6597 6607 6617 6624 6631 6641 6643.8 6643.18 6644
. . . . . . . . .
AN1-EnvB ttaaccccac tctgtgttac tttaaattgc actgatgatt tgaggactaa tgctactaat accaccaata gtagtgctac cactaatacc [450]
L T P L C V T L N C T D D L R T N A T N T T N S S A T T N T [150]
6654 6664 6674.3 6681 6691 6701 6711 6721 6731
. . . . . . . . .
AN1-EnvB actagtagtg gggggggaac gatggagggg gaaaaaggag aaataaaaaa ctgctctttc aatgtcacca caagcataag agataagatg [540]
T S S G G G T M E G E K G E I K N C S F N V T T S I R D K M [180]
6741 6751 6761 6771 6781 6789.2 6789.12 6793 6803
. . . . . . . . .
AN1-EnvB cagaaagaat atgcactttt ttataaactt gatgtagtac caatagataa tgataataat aacactaata ataataccag ctataggttg [630]
Q K E Y A L F Y K L D V V P I D N D N N N T N N N T S Y R L [210]
6813 6823 6833 6843 6853 6863 6873 6883 6893
. . . . . . . . .
AN1-EnvB ataaattgta acacctcagt cattacacag gcctgtccaa aggtatcctt tgagccaatt cccatacatt attgtacccc ggctggtttt [720]
I N C N T S V I T Q A C P K V S F E P I P I H Y C T P A G F [240]
6903 6913 6923 6933 6943 6953 6963 6973 6983
. . . . . . . . .
AN1-EnvB gcgattctaa agtgtaatga taagaagttc aatggaacag gaccatgtac aaatgtcagc acagtacaat gtacacatgg aattaggcca [810]
A I L K C N D K K F N G T G P C T N V S T V Q C T H G I R P [270]
6993 7003 7013 7023 7033 7043 7053 7063 7073
. . . . . . . . .
AN1-EnvB gtagtgtcaa ctcaactgct gttaaatggc agtctagcag aagaagaggt agtaattaga tctgaaaatt tcacggacaa tgctaaaacc [900]
V V S T Q L L L N G S L A E E E V V I R S E N F T D N A K T [300]
7083 7093 7103 7113 7123 7133 7143 7157 7169
. . . . . . . . .
AN1-EnvB ataatagtac agctgaatga atctgtagaa attaattgta caagacccaa caacaataca agaaaaagta tacctatagg accagggaga [990]
I I V Q L N E S V E I N C T R P N N N T R K S I P I G P G R [330]
7179 7189 7196 7206 7216 7226 7236 7246 7256
. . . . . . . . .
AN1-EnvB gcactttatg caacaggaaa aataatagga gatataagac aagcacattg taaccttagt agagcaaaat ggaataacac tttaaaacag [1080]
A L Y A T G K I I G D I R Q A H C N L S R A K W N N T L K Q [360]
7266 7276 7286 7296 7303 7313 7323 7333 7343
. . . . . . . . .
AN1-EnvB atagttacaa aattaagaga acaatttggg aataataaaa caacaatagt ctttaatcaa tcctcaggag gggacccaga aattgtaatg [1170]
I V T K L R E Q F G N N K T T I V F N Q S S G G D P E I V M [390]
7353 7363 7373 7383 7393 7403 7410 7420 7426.4
. . . . . . . . .
AN1-EnvB cacagtttta attgtggagg ggaatttttc tactgtaatt caacacaact gtttaatagt acttggcatt ttaatggtac ttgggggaat [1260]
H S F N C G G E F F Y C N S T Q L F N S T W H F N G T W G N [420]
7431 7441 7451 7458 7468 7478 7488 7498 7508
. . . . . . . . .
AN1-EnvB aataatactg aaaggtcaaa taacgctgca gatgataatg acactatcac actcccatgc agaataaaac aaattataaa catgtggcag [1350]
N N T E R S N N A A D D N D T I T L P C R I K Q I I N M W Q [450]
7518 7528 7538 7548 7558 7568 7578 7588 7598
. . . . . . . . .
AN1-EnvB gaagtaggaa aagcaatgta tgcccctccc atcagtggac aaattagatg ttcatcaaat attacagggc tactattaac aagagatggt [1440]
E V G K A M Y A P P I S G Q I R C S S N I T G L L L T R D G [480]
7607.1 7609 7616 7626 7636 7646 7656 7666 7676
. . . . . . . . .
AN1-EnvB ggtaataatg agaacacgaa caataccgac actgagatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat [1530]
G N N E N T N N T D T E I F R P G G G D M R D N W R S E L Y [510]
7686 7696 7706 7716 7726 7736 7746 7756 7766
. . . . . . . . .
AN1-EnvB aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga [1620]
K Y K V V K I E P L G V A P T K A K R R V V Q R E K R A V G [540]
7773 7783 7793 7803 7813 7823 7833 7843 7853
. . . . . . . . .
AN1-EnvB atgctaggag ctatgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa [1710]
M L G A M F L G F L G A A G S T M G A A S M T L T V Q A R Q [570]
7863 7873 7883 7893 7903 7913 7923 7933 7943
. . . . . . . . .
AN1-EnvB ttattgtctg gtatagtgca acagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc [1800]
L L S G I V Q Q Q N N L L R A I E A Q Q H L L Q L T V W G I [600]
7953 7963 7973 7983 7993 8003 8013 8023 8033
. . . . . . . . .
AN1-EnvB aaacagctcc aggcaagagt cctggctgtg gaaagatacc taaaggatca acagctccta gggatttggg gttgctctgg aaaactcatc [1890]
K Q L Q A R V L A V E R Y L K D Q Q L L G I W G C S G K L I [630]
8043 8053 8063 8073 8083 8093 8103 8113 8123
. . . . . . . . .
AN1-EnvB tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga taagatttgg aataacatga cctggatgga gtgggaaaga [1980]
C T T A V P W N A S W S N K S L D K I W N N M T W M E W E R [660]
8133 8143 8153 8163 8173 8183 8193 8203 8213
. . . . . . . . .
AN1-EnvB gaaattgaca attacacagg cttaatatac accttaattg aagaatcgca gaaccaacaa gaaaagaatg aacaagaatt attggaattg [2070]
E I D N Y T G L I Y T L I E E S Q N Q Q E K N E Q E L L E L [690]
8223 8233 8243 8253 8263 8273 8283 8293 8303
. . . . . . . . .
AN1-EnvB gataagtggg caagtttgtg gaattggttt gacataacaa actggctgtg gtatataaaa atattcataa tgatagtagg aggcttggta [2160]
D K W A S L W N W F D I T N W L W Y I K I F I M I V G G L V [720]
8313 8323 8333 8343 8353 8363 8373 8383 8393
. . . . . . . . .
AN1-EnvB ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatactca ccattatcgt ttcagacccg cctcccagcc [2250]
G L R I V F A V L S I V N R V R Q G Y S P L S F Q T R L P A [750]
8403 8413 8423 8433 8443 8453 8463 8473 8483
. . . . . . . . .
AN1-EnvB ccgaggggac ccgacaggcc cgaaggaatc gaagaagaag gtggagagag agacagagac agatccggtc gattagtgaa tggattctta [2340]
P R G P D R P E G I E E E G G E R D R D R S G R L V N G F L [780]
8493 8503 8513 8523 8533 8543 8553 8563 8573
. . . . . . . . .
AN1-EnvB gcacttatct gggacgacct gcggagcctg tgcctcttca gctaccaccg cttgagagac ttactcttga ttgtagcgag gattgtggaa [2430]
A L I W D D L R S L C L F S Y H R L R D L L L I V A R I V E [810]
8583 8593 8603 8613 8623 8633 8643 8653 8663
. . . . . . . . .
AN1-EnvB cttctgggac gcagggggtg ggaagccctc aaatattggt ggaatctcct gcagtattgg agtcaggaac taaagaatag tgctgttagc [2520]
L L G R R G W E A L K Y W W N L L Q Y W S Q E L K N S A V S [840]
8673 8683 8693 8703 8713 8723 8733 8743 8753
. . . . . . . . .
AN1-EnvB ttgcttaatg ccacagctat agcagtagct gaggggacag atagggttat agaagtagta caaagagctt gtagagctat tctccacata [2610]
L L N A T A I A V A E G T D R V I E V V Q R A C R A I L H I [870]
8763 8773 8783 8793
. . . .
AN1-EnvB cctagaagaa taagacaggg cttagaaagg gctttgctat aa [2652]
P R R I R Q G L E R A L L * [883]
FIG 2. Subtype B MRCA: Nucleotide and inferred amino acid
sequence of AN1env. Numbers above the alignment indicate positions
relative to the sequence of HIV HXB2 (GenBank accession number K03455).