SOP for Acrylamide Use


University of Washington Department of Microbiology

Standard Operating Procedures for Chemicals or Processes

#1   Process

(if applicable)

Use of Acrylamide.  Use in polyacrylamide gels.

#2  Chemicals

Un-polymerized acrylamide is toxic (neurotoxin) and suspect carcinogen.

#3   Personal Protective                        Equipment (PPE)

Double layers of nitrile gloves, lab coat, and goggles required when handling the solid powder.  Use diapers when pouring gels.

#4   Environmental /

Ventilation Controls

Handle powder inside the designated fume hood located in 352.

#5  Special Handling Procedures & Storage Requirements

Avoid getting the unpolymerized acrylamide on skin, gloves, clothing, etc.

#6  Spill and Accident                         Procedures

If skin contact is made, wash copiously with water.  Call Poison Control Center if necessary.  Absorb spill with diatomaceous earth and call EH&S at 543-0467 for further information.

#7  Waste Disposal

Polymerized acrylamide is non-toxic and can be disposed in the trash.  Small amounts of unpolymerized liquid can be poured down the sink with copious amounts of water.

#8  Special Precautions                       for Animal Use

(if applicable)


Particularly hazardous

substance involved?

  X   YES:

Blocks #9 to #11 are Mandatory


Blocks #9 to #11 are Optional.

#9  Approval Required

Users must receive specific process training and information about acrylamide from their supervisor before being authorized to perform procedures.

#10 Decontamination

Double nitrile gloves.  Use copious amounts of soap and water.

#11 Designated Area

Inside fume hood between microscope and water bath.

Name:                                                                                  Title:

Signature:                                                                                              Date:                                    

Environmental Health and Safety, Box 354400                        



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