SOP for Benzene Use


University of Washington Department of Microbiology

Standard Operating Procedures for Chemicals or Processes

#1   Process

(if applicable)

Injections of Benzene Standards for Gas Chromatograph Calibrations and Sample Analyses (Note:  All use of benzene is strictly regulated by occupational health regulation WAC 296-62-07523.)

#2  Chemicals

Benzene, at known and unknown concentrations.

#3   Personal Protective                        Equipment (PPE)

Chemical splash goggles, butyl or natural rubber gloves, and a lab coat or apron is required.

#4   Environmental /

Ventilation Controls

Benzene-containing solutions should be dispensed and used only in a fume hood.  Syringe purging done in fume hood.

#5  Special Handling Procedures & Storage Requirements

Mixing and dispensing done in an operating fume hood with all sources of ignition turned off (hot plates, burners, etc.).  Benzene stored in metal safety cans or glass bottles (1 liter maximum) as much as possible.  Transported in spill-proof carriers.  Benzene is stored in a flammable cabinet, separate from acids, bases, and oxidizers.  The flammable cabinet is located *


#6  Spill and Accident                         Procedures

Try to stop spill if spill is on-going.  If splash on skin occurs, wash immediately with soap and water, and remove any contaminated apparel while washing.  Call 9-911 in the event of a spill beyond lab’s capabilities.  Remove all sources of ignition from the spill area.  Respiratory protection is required to clean up spills outside of fume hoods (and respirator users must be properly certified).  Use absorbent pads or vermiculite to clean up small fume hood spills or to dike larger spills.  Absorbent pads are stored at *


After clean-up, room air must be monitored by EH&S prior to re-occupancy.

#7  Waste Disposal

Place used absorbent in metal can with leak-proof lid.  Over-pack with additional absorbent.  Seal can and label with “Hazardous Waste Label” and contact EH&S (685-2848) for collection.

#8  Special Precautions                       for Animal Use

(if applicable)


Particularly hazardous

substance involved?

  X   YES:

Blocks #9 to #11 are Mandatory


Blocks #9 to #11 are Optional.

#9  Approval Required

Users must receive specific physical and health hazard information and safe laboratory work practices training from their supervisor.  Representative breathing zone air sampling shall be taken to ensure that exposures do not exceed regulated levels.  (Contact EH&S for additional information.)

#10 Decontamination

Immediately wash with soap and water.

#11 Designated Area

Room *                                      .  Special signage may be required depending on air sampling results.

Name:                                                                                  Title:

Signature:                                                                                              Date:                                     

Environmental Health and Safety, Box 354400                                             


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