SOP for Chloroform Use


University of Washington Department of Microbiology

Standard Operating Procedures for Chemicals or Processes

#1   Process

(if applicable)

Handling, use and storage of chloroform for molecular biology.

#2  Chemicals

May be fatal if inhaled, swallowed, or absorbed through skin.  Irritating to eyes, mucous membranes, and upper respiratory tract. Causes skin irritation. May be carcinogenic.  Exposure to fire or high temperatures may lead to formation of phosgene, a highly toxic gas.

#3   Personal Protective                        Equipment (PPE)

Always where gloves.  Nitrile gloves have a 4 minute breakthrough time. Use lab coat and eye protection also.

#4   Environmental /

Ventilation Controls

Handle chloroform in hoods located in either 351 of 352.

#5  Special Handling Procedures & Storage Requirements

Store tightly closed  in cool dark place (dark glass).  Incompatible with STRONG BASES

Wash well after handling


#6  Spill and Accident                         Procedures

For large spill, call for someone with SCUBA apparatus (685-2848). Absorb liquid with spillX-S or diatomaceous earth and contain in plastic pail or glass bottle.

Exposure:  Nausea, dizziness, and headache.

In the event of skin contact, immediately wash with soap and water. In case of eye contact, promptly wash with copious amounts of water for 15 minutes and obtain medical attention.

#7  Waste Disposal

Place in appropriate container, labeled with Haz water label and contact EH&S (685-2848) for collection.

#8  Special Precautions                       for Animal Use

(if applicable)


Particularly hazardous

substance involved?


Blocks #9 to #11 are Mandatory

  X    NO:

Blocks #9 to #11 are Optional.

#9  Approval Required


#10 Decontamination

Solvent wash area with 60-70% ethanol followed by washing with soap and water solution.

#11 Designated Area

Inside fume hood only.

Name:                                                                                  Title:

Signature:                                                                                              Date:                                    

Environmental Health and Safety, Box 354400                        



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