SOP for Flammable Solvents Use


University of Washington Department of Microbiology

Standard Operating Procedures for Chemicals or Processes

#1   Process

(if applicable)

Flammable Solvents- use and storage - including the following: methanol, pipendine, ethyl alcohol, n-butanol, xylenes, ethanolamine, formaldehyde, iso-amyl alcohol, dimethylformamide, n,n-dimethyformaldehyde, triethanolamine, glycerol, isopropanol, dimethyl sulfoxide, chloroform, phenol, and temed.

#2  Chemicals

Flammable solvent vapors can travel and can produce fire and explosion if an ignition source is contacted. Some flammable solvents are more hazardous than others. Many solvents also have an effect on the central nervous system and at high concentrations cause sedation, coma and death. Contact with solvents can de-fat skin and cause irritation of skin and mucous membranes.

#3   Personal Protective                        Equipment (PPE)

Wear chemical splash goggles, consult Appendix C for proper glove selection. Call EH&S (3-7388) for further information. A lab coat or apron is recommended for personal protection and is required when dispensing or cleaning up spill quantities greater than 1 liter.

#4   Environmental /

Ventilation Controls

Solvents should be dispensed only in a fume hood or in a well ventilated space which has been approved and permitted by the Seattle Fire Department.

#5  Special Handling Procedures & Storage Requirements

Mixing or dispensing should be done in a hood with all sources of ignition eliminated (hot plates, burners, etc.). Store in metal safety cans whenever possible. Solvents should be stored in appropriate flammable cabinets, separate from acids, bases, and oxidizers. Flammable cabinets located in rooms 351 and 352 chemical fume hood base cabinets.

#6  Spill and Accident                         Procedures

Remove all sources of ignition from the spill area if it is safe to do it. Small fires may be extinguished if it is safe and the operator is trained to use the fire extinguisher. Wipe down spill area with solvent absorbent pads. Solvent absorption pads can be found next to bookcase in 352.

#7  Waste Disposal

Place in an appropriate container, label with "Hazardous Waste Label" and contact Environmental Health and Safety (5-2848) for collection.

PDo Not evaporate flammable solvents in the fume hood.

#8  Special Precautions                       for Animal Use

(if applicable)


Particularly hazardous

substance involved?


Blocks #9 to #11 are Mandatory

  X    NO:

Blocks #9 to #11 are Optional.

#9  Approval Required


#10 Decontamination


#11 Designated Area


Name:                                                                                  Title:

Signature:                                                                                              Date:                                    

Environmental Health and Safety, Box 354400                                 




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