SOP for Inorganic Acid Use


University of Washington Department of Microbiology

Standard Operating Procedures for Chemicals or Processes

#1   Process

(if applicable)

Inorganic Acids - handling, dispensing, and diluting acids including: phosphoric acid, sulfuric acid and hydrochloric acid (see attached sheet).

#2  Chemicals

Acids cause burns to skin and eyes upon contact and to mucous membranes if inhaled or ingested.

#3   Personal Protective                        Equipment (PPE)

Wear chemical splash goggles and heavy-duty neoprene gloves for concentrated acids. For diluted acids greater than pH 2, use nitrile gloves. A lab coat or apron is recommended for personal protection.

#4   Environmental /

Ventilation Controls

Concentrated acids should be dispensed in a fume hood.

#5  Special Handling Procedures & Storage Requirements

When diluting acids, small amounts should be added gradually to water and mixed thoroughly to dissipate any heat generated. Inorganic and organic acids should be stored in separate bins in the acid storage cabinets. Acids should be stored separately from bases, oxidizers and flammable solvents. Acids in glass bottles over 1 liter should be transported in spill proof carriers. Acids are stored in 352 hood base cabinet.

#6  Spill and Accident                         Procedures

In case of skin contact, flush affected areas with copious amounts of water for 15 minutes. Obtain medical attention. Neutralize any spilled acids with sodium bicarbonate or spill pads to clean up. Spill kit can be found next to bookcase in 352 .

#7  Waste Disposal

Small quantities may be neutralized (see Appendix F). Acids with pH <2 are a hazardous waste and should be labeled with a 'Hazardous Waste Label'. Contact EH&S at 5-2848 for collection.

#8  Special Precautions                       for Animal Use

(if applicable)


Particularly hazardous

substance involved?


Blocks #9 to #11 are Mandatory

  X    NO:

Blocks #9 to #11 are Optional.

#9  Approval Required


#10 Decontamination


#11 Designated Area


Name:                                                                                  Title:

Signature:                                                                                              Date:                                    

Environmental Health and Safety, Box 354400                         *to be filled in by PI or Supervisor



See Disclaimer