SOP for Petroleum Ether


University of Washington Department of Microbiology

Standard Operating Procedures for Chemicals or Processes

#1   Process

(if applicable)

Use of petroleum ether for molecular biology techniques

#2  Chemicals

Petroleum ether is a toxic and flammable liquid. Its vapor may travel considerable distance to source of ignition and flash back. Container explosion may occur under fire conditions.

#3   Personal Protective                        Equipment (PPE)

Wear chemical-resistant gloves, safety goggles, and other protective clothing. 

#4   Environmental /

Ventilation Controls

Use in the chemical fume hood located in either 351 or 352 .

#5  Special Handling Procedures & Storage Requirements

Store in hazardous chemical storage room.. Use only in a fume hood with all sources of ignition eliminated.


#6  Spill and Accident                         Procedures

Remove all sources of ignition from the spill area. (over area with an activated carbon absorbent, take up and place in closed container, and transport outdoors. Ventilate area and wash spill site after material pick up is complete. In case of contact with skin or eyes, wash copiously with water for 15 minutes. Kit located on bookcase 352.  

#7  Waste Disposal

Place in an appropriate container, label with “Hazardous Waste Label” and contact EH & S (5-2848) for collection.

#8  Special Precautions                       for Animal Use

(if applicable)


Particularly hazardous

substance involved?

 X    YES:

Blocks #9 to #11 are Mandatory


Blocks #9 to #11 are Optional.

#9  Approval Required

Approval not required.

#10 Decontamination

Use copious amounts of soap and water after material is absorbed.

#11 Designated Area

Inside fume hood only.

Name:                                                                                 Title:

Signature:                                                                                            Date:                                    

Environmental Health and Safety, Box 354400                                               




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