SOP for Toluene


University of Washington Department of Microbiology

Standard Operating Procedures for Chemicals or Processes


#1   Process

(if applicable)

Use of Toluene for organic chemistry related to molecular biology.

#2  Chemicals

Toluene is toxic and extremely flammable. Its vapor may travel considerable distance to source of ignition and flash back. Container explosion may occur under fire conditions.


#3   Personal Protective                        Equipment (PPE)

Wear safety glasses, chemical-resistant gloves, and lab coat or apron for personal protection.


#4   Environmental /

Ventilation Controls

Use only in the chemical fume hoods located in  351 or 352.


#5  Special Handling Procedures & Storage Requirements

Store in chemical hood base cabinets or hazardous materials storage room. Use in a fume hood with al sources of ignition eliminated.



#6  Spill and Accident                         Procedures

Remove all sources of ignition from the spill area. Cover area with an activated carbon absorbent, place in closed container, and transport outdoors. Ventilate area and wash spill site after material pick up is complete. In case of contact with skin or eyes, wash with copious amounts of  water for 15 minutes. The spill kit is located next to the bookcase in 352.


#7  Waste Disposal

Place in an appropriate container, label with “ Hazardous Waste Label” and contact EH & S (5-2848) for collection.


#8  Special Precautions                       for Animal Use

(if applicable)



Particularly hazardous

substance involved?

 X   YES:

Blocks #9 to #11 are Mandatory



Blocks #9 to #11 are Optional.


#9  Approval Required

No approval required.


#10 Decontamination

Use copious amounts of soap and water after material is  absorbed. 


#11 Designated Area

Inside fume hood only.


Name:                                                                                 Title:


Signature:                                                                                            Date:                                    


Environmental Health and Safety, Box 354400                                   




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