Lab Protocols Index
Data Analysis
Flow Cytometry
Chemokine Receptor Expression Assay by Flow Cytometry
Intracellular p24 Assay by Flow Cytometry (BD)
Intracellular p24 Assay by Flow Cytometry (Caltag)
Microarrays
Agilent Bioanalyzer RNA Samples
Hot Asymmetric PCR (Katze Lab)
Katze Lab Total RNA Extraction Method
Mullins Lab Total RNA Extraction
Oligonucleitide Hybridization to Glass Arrays
Preparation of Linear Acrylamide
RNA Extraction with Qiagen RNEASY Midi Kit
Radiolabeled First-Strand cDNA Synthesis
Radiolabeling DNA Probe for Northerns
Molecular Biology
Isolation of Chromosomal DNA from Mammalian Cells
PCR Conditions (96-Well Format)
Purification of PCR Products (96-wells Format)
Replication of cDNA Clones (96-Wells Format)
TOPO T/A Cloning of PCR Products
Other
Tissue Culture (BL2)
PBMC Isolation by Ficoll Gradient
Passaging Mammalian Cell Lines
Protocol for Freezing Mammalian Cells
Protocol for Thawing Mammalian Cells
Tissue Culture (BL3)
BL3 Standard Operating Procedures
Determining Coreceptor Usage of HIV-1 on U87 Glioma Cells
Pairwise growth competition assay