Fetch files from CFAR DSF ftp-site

Start SEQUENCHER

Drag sequences to SEQUENCHER window

Open files by double clicking

Only use files with good signal

Find restriction site of vector (eg. EcoRI GAATTC) with FIND option

Crop vector sequence

Open chromatogram

Find where good sequence ends or poly A tail starts

Crop rest of sequence

Export sequence as Pearson text

One file:

• copy sequence into window at NCBI webpage

• do basic blastn nr search

Multiple files:

• start SEARCH LAUNCHER by dropping sequence files onto the camel symbol

• type DNA

• type 10 (BLASTN NR) or 12 (dbEST)

• results are returned into the SEARCH LAUNCHER directory