Mullins Molecular Retrovirology Lab

  • Department of Microbiology
  • School of Medicine
  • University of Washington
University of Washington/Fred Hutch Center for AIDS Research

Sequence Analysis for Research Genetics Clones

Fetch files from CFAR DSF ftp-site


Drag sequences to SEQUENCHER window

Open files by double clicking

Only use files with good signal

Find restriction site of vector (eg. EcoRI GAATTC) with FIND option

Crop vector sequence

Open chromatogram

Find where good sequence ends or poly A tail starts

Crop rest of sequence

Export sequence as Pearson text

One file:

  • copy sequence into window at NCBI webpage

  • do basic blastn nr search

Multiple files:

  • start SEARCH LAUNCHER by dropping sequence files onto the camel symbol

  • type DNA

  • type 10 (BLASTN NR) or 12 (dbEST)

  • results are returned into the SEARCH LAUNCHER directory