Sequence Analysis for Research Genetics Clones
Separation of T-Cell Subsets by FACStar
Measurement of Intracellular Calcium By Flow Cytometry
Chemokine Receptor Expression Assay by Flow Cytometry
Intracellular p24 Assay by Flow Cytometry (BD)
Intracellular p24 Assay by Flow Cytometry (Caltag)
Radiolabeled First-Strand cDNA Synthesis
Oligonucleitide Hybridization to Glass Arrays
Hot Asymmetric PCR (Katze Lab)
Radiolabeling DNA Probe for Northerns
Agilent Bioanalyzer RNA Samples
Preparation of Linear Acrylamide
Selection of Poly A+ RNA (Katze Lab w/modifications)
mRNA Isolation with Qiagen OligoTex Kit
Katze Lab Total RNA Extraction Method
Mullins Lab Total RNA Extraction
RNA Extraction with Qiagen RNEASY Midi Kit
Isolation of Chromosomal DNA from Mammalian Cells
TaqMan Assays on Demand Preparation and Real Time PCR
TOPO T/A Cloning of PCR Products
Purification of PCR Products (96-wells Format)
PCR Conditions (96-Well Format)
Replication of cDNA Clones (96-Wells Format)
WALKER WHOLE GENOME SUBTYPE C PROTOCOLS
PBMC Isolation by Ficoll Gradient
Passaging Mammalian Cell Lines
Protocol for Freezing Mammalian Cells
Protocol for Thawing Mammalian Cells
TC Supplies for Mullins TC Lab (Rosen 344)
Ten Golden Rules of Tissue Culture
Pairwise growth competition assay
Determining Coreceptor Usage of HIV-1 on U87 Glioma Cells
