Mullins Molecular Retrovirology Lab

  • Department of Microbiology
  • School of Medicine
  • University of Washington
University of Washington/Fred Hutch Center for AIDS Research

Ten Golden Rules of Tissue Culture

1.   No gloves in regular trash.

2.   Seal off vacuum when done.

3.   Empty vacuum trap flask when full.

4.   Fill bleach bottle when empty

5.   Clean hemocytometer after use.

6.   No liquid in biohazard bag.

7.   Put glass pipettes fully in sharps container.

8.   Fold new pipette boxes when full.

9.   Discard full pipette boxes.

10.   Keep opened tip boxes sterile