Mullins Molecular Retrovirology Lab

  • Department of Microbiology
  • School of Medicine
  • University of Washington
University of Washington/Fred Hutch Center for AIDS Research

Lab Protocols Index


Agilent Bioanalyzer RNA Samples

Aminoallyl labeling

CY-DYE PROBE Synthesis

Eberwine T7 RNA Amplification

Hot Asymmetric PCR (Katze Lab)

In Vitro Transcription

Katze Lab Total RNA Extraction Method

Mullins Lab Total RNA Extraction

Northern Transfer

Oligonucleitide Hybridization to Glass Arrays

Preparation of Linear Acrylamide

Processing Q-Bot Membranes

Quantitative PCR

RNA Extraction with Qiagen RNEASY Midi Kit

RNA-Gel for Northern Transfer


Radiolabeled First-Strand cDNA Synthesis

Radiolabeling DNA Probe for Northerns

Selection of Poly A+ RNA (Katze Lab w/modifications)

mRNA Isolation with Qiagen OligoTex Kit